The present research aims to design a model for the creation and development of knowledge-based cooperative companies in Semnan province by a mixed qualitative-quantitative approach. The qualitative approach was based on grounded theory and the quantitative approach was based on structural equation analysis. In terms of purpose, this research is an applied study, and in terms of method, it is a descriptive survey study. The population includes the knowledge-based companies of Semnan province. Out of the mentioned data collection was done by library studies and semi-structured interviews in the qualitative phase and questionnaire in the quantitative phase. In the qualitative phase, the factors and the data obtained from the interviews were analyzed by Atlas.ti8 and grounded theory coding proposed by Strass and Corbin. The components and indicators of the creation and development of knowledge-based cooperative companies were identified on this basis. In the quantitative phase, Lisrel software and IBM SPSS statistics.26 software were used to apply the interpretive structural equation for developing the final research model. The findings include the indicators of components of creation, and development of knowledge-based cooperative companies in Semnan province and the model proposed for this purpose. The most important of these components: Education and research, Technology, Management strategies and policy-making, New platforms and infrastructures, Expansion of knowledge application, Knowledge-based innovation and creativity.